The Banisteriopsis pubipetala A. Juss. came to be considered synonymous with Diplopterys pubipetala (A. Juss.) W.R. Anderson and C. Davis. D. pubipetala is a liana, occurring in the cerrado biome, belonging to a genus already described in the literature for the antioxidant action of some species and for being effective in controlling some types of cancer, including cutaneous melanoma, which is the most common malignant tumor in the whole human species. However, D. pubipetala is still poorly studied with regard to bioactive potential and chemical composition. The main objective of the work is to evaluate antitumor activity by evaluating migratory activity, detecting cell death and determining cell viability of the partitions and fractions of D. pubipetala leaf and stem. The fractions demonstrated the ability to induce death, decrease cell viability and even suppress B16F10 migration. This study is a pioneer in the antitumor evaluation of D. pubipetala and opens new lines of research for exploration. This demonstrates the promising possibility of using the species to develop new strategies for the treatment and prevention of cutaneous melanoma.